User:Meng Xiao He/Notebook/fall08/2008/11/04

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Test digest w/ SmaI

11.4.puc19 stuff.mx.jpg


  • left most: pUC19 digest, control (2686)
  • next 3: cbb3+pUC19
  • 1kb ladder
  • next 2: cco+pUC19
  • 100bp ladder
  • next 3: Duo+pUC19
  • lane 12 is good> it should contain the Duo flank in pUC19's XmaI site

New transformations

  • TOPO things into TOP10
  • pER21 (+0, cbb3, cco, Duo) into chem pir-116