User:Meng Xiao He/Notebook/fall08/2008/11/05

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Transformation results

  • Whatever was spread as X-gal seems to have been toxic
  • Labels were not distinguishable- so don't know which of the TOPO/pER21 cluster is which
  • TOPO cloning much better this time
    • Hundreds on each plate
  • Chem pir-116 are competent it seems
    • pER21 retransformed and grew
    • 2ul pUC19 control from Invitrogen gave hundreds of colonies
    • Ligations w/ pER21 and flanks gave a few colonies each (<15)- 3 picked from each plate into liquid culture