User:Mbennie/Notebook/Papers and Books/Autotransporters General

1. Jose J. Autodisplay: efficient bacterial surface display of recombinant proteins. Appl Microbiol Biotechnol. 2006 Feb;69(6):607-14. DOI:10.1007/s00253-005-0227-z |

• Focuses on AIDA-I protein which is an an autotransporter naturally expressed in e coli
• Discusses applications using AIDA-1: expressing enzymes(whole cell biocatalysis), enzyme inhibitors(library screening), and epitotes(vaccine development)
• Interesting note: passenger proteins can dimerize on the cell surface

2. Rutherford N and Mourez M. Surface display of proteins by gram-negative bacterial autotransporters. Microb Cell Fact. 2006 Jun 20;5:22. DOI:10.1186/1475-2859-5-22 |

• Autotransporter = N-terminal signal peptide + passenger domain + translocator domain
• Study of autotransporters encourage because of their simplicity
• Possible issues with autotransporters
  • Passenger must be in "translocation-competent state" in periplasm
  • Need specific signal sequences
  • Host can introduce genetic background
• Other systems: Omp85, LamB, OmpA, PhoE, OmpC, TraT, FhuA, and intimin
• Autotransporters (AT) belong to type V secretion system, specifically the type Va branch
• Form beta-barrel to stabilize protein in the outer membrane
• Passenger can undergo some level of folding in the periplasm
• Most passenger domains are cleaved into media
• "System is not amenable to the display of eukaryotic cDNA libraries."
• 50,000 - 100,000 copies per cell

3. Henderson IR, Navarro-Garcia F, and Nataro JP. The great escape: structure and function of the autotransporter proteins. Trends Microbiol. 1998 Sep;6(9):370-8. DOI:10.1016/s0966-842x(98)01318-3 |

• "No energy coupling or accessory factors are known to be required for the translocation process"
• Some interesting autotransporters in e coli