User:Mbennie/Notebook/Papers and Books/Applications

1. Anderson JC, Clarke EJ, Arkin AP, and Voigt CA. Environmentally controlled invasion of cancer cells by engineered bacteria. J Mol Biol. 2006 Jan 27;355(4):619-27. DOI:10.1016/j.jmb.2005.10.076 |

• Used invasin to target tumor cells using factors such as hypoxia and bacteria's natural tendency to grow in regions of high cell density
• Mammalian cells normally take up a cell with invasin
• Used directed evolution to find strains that worked according to their parameters: invasion under certain condition and no invasion otherwise

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• Isolated phage that were targeted towards specific organs by repeated injection and recovery
• Found phage that were 8 times more targeted towards the kidneys or brain

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• Attempts to express MT in e coli and secreted into media in order to bind Cu
• MT is a really cool protein (binds several types of heavy metals)
• Hypothesized that metal binds to MT inside of cell, but in periplasm metal release when disulfide bridge is formed
• Removed 34% of Cu using best plasmid