User:Mbennie/Notebook/Papers and Books/Applications

1. Anderson JC, Clarke EJ, Arkin AP, and Voigt CA. Environmentally controlled invasion of cancer cells by engineered bacteria. J Mol Biol. 2006 Jan 27;355(4):619-27. DOI:10.1016/j.jmb.2005.10.076 |

• Used invasin to target tumor cells using factors such as hypoxia and bacteria's natural tendency to grow in regions of high cell density
• Mammalian cells normally take up a cell with invasin
• Used directed evolution to find strains that worked according to their parameters: invasion under certain condition and no invasion otherwise

2. Pasqualini R and Ruoslahti E. Organ targeting in vivo using phage display peptide libraries. Nature. 1996 Mar 28;380(6572):364-6. DOI:10.1038/380364a0 |

• Isolated phage that were targeted towards specific organs by repeated injection and recovery
• Found phage that were 8 times more targeted towards the kidneys or brain

3. Cols N, Roepstorff K, Gonzàlez-Duarte R, and Atrian S. Secretion of mouse-metallothionein by engineered E. coli cells in metal-enriched culture media. J Mol Microbiol Biotechnol. 2001 Oct;3(4):507-12.

• Attempts to express MT in e coli and secreted into media in order to bind Cu
• MT is a really cool protein (binds several types of heavy metals)
• Hypothesized that metal binds to MT inside of cell, but in periplasm metal release when disulfide bridge is formed
• Removed 34% of Cu using best plasmid