User:Mbennie/Notebook/Lab Notebook/Notebook/2007/08/05

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Cellular Adhesion

  • Glycerol
    • Took 1.6ml of liquid culture and created 10% glycerol stocks
      • Signal Sequence #2
      • Signal Sequence #3
      • IgAbc #1
      • Fos #1
      • JunB #3
      • GCN4 #2
  • Miniprep
    • Extracted DNA from remaining liquid culture (~6ml)
    • Eluted in 50ul water
  • Spec
    • Signal Sequence #2: 29.2 ng/ul
    • Signal Sequence #3: 26.4 ng/ul
    • IgAbc #1: 27.7 ng/ul
    • Fos #1: 27.3 ng/ul
    • JunB #3: 30.9 ng/ul
    • GCN4 #2: 28.2 ng/ul
  • Sequencing
    • Template: 8ul DNA, 1.8ul water, .2ul primer (all samples with VF2 and IgAbc with VR)
  • Liquid Culture
    • Grew up cultures in 8ml Amp/Cl LB that didn't work last night (cells died in water) with colony PCR backup plate
      • Fos #3
      • JunB #2