User:Mbennie/Notebook/Lab Notebook/Notebook/2007/08/05

Cellular Adhesion

• Glycerol
  • Took 1.6ml of liquid culture and created 10% glycerol stocks
    • Signal Sequence #2
    • Signal Sequence #3
    • IgAbc #1
    • Fos #1
    • JunB #3
    • GCN4 #2

• Miniprep
  • Extracted DNA from remaining liquid culture (~6ml)
  • Eluted in 50ul water

• Spec
  • Signal Sequence #2: 29.2 ng/ul
  • Signal Sequence #3: 26.4 ng/ul
  • IgAbc #1: 27.7 ng/ul
  • Fos #1: 27.3 ng/ul
  • JunB #3: 30.9 ng/ul
  • GCN4 #2: 28.2 ng/ul

• Sequencing
  • Template: 8ul DNA, 1.8ul water, .2ul primer (all samples with VF2 and IgAbc with VR)

• Liquid Culture
  • Grew up cultures in 8ml Amp/Cl LB that didn't work last night (cells died in water) with colony PCR backup plate
    • Fos #3
    • JunB #2