User:Matthew R Skorski/Notebook/471 - Exp BioChem/2015/12/02

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Objective

To run the experiments to analyze proteinase k at varying concentrations using ICP, conductivity, and UV-VIS.

Description

The fibers were spun down at high speeds in a falcon tube. 3 mL were taken for UV-VIS analysis and the rest for conductivity and ICP.

  1. Conductivity
    1. The fiber samples and supernatants were analyzed using the lab conductivity probles
  2. ICP
    1. The fiber samples and supernatants were analyzed using the ICP machine that Dr. Hartings had set up
  3. UV-VIS
    1. The fiber samples and supernatants were analyzed using the ocean optics machine.

Results

Conductivity 12 2 Conductivity.png

ICP 12 2 ICP.png

UV-VIS

1 uM, 100 nM, 10 nM, and 1 nM Proteinase-K Digestion of AuNP Fibers .png

Concentration vs. Average Peak Absorbance at 532 nm .png