User:Matthew R Skorski/Notebook/471 - Exp BioChem/2015/12/01

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Objective

To set up the experiments to analyze proteinase k at varying concentrations using ICP, conductivity, and UV-VIS.

Description

  1. Gold Standards for ICP
    1. Standards were made with DI water and 1 mg/ml gold standard
    2. Concentrations of standards were 0.1 mg/mL, 0.01 mg/mL, 0.001 mg/mL, 0.0001 mg/mL, 0.00001 mg/mL, and 0.000001 mg/mL
  2. Fiber Samples
    1. 12 20 mL samples
      1. Tubes 1-3: 1 uM proteinase-K
      2. Tubes 4-6: 100 nM proteinase-K
      3. Tubes 7-9: 10 nM proteinase-K
      4. Tubes 10-12: 1 nM proteinase-K
    2. Samples were left to digest overnight
    3. Supernatant from fiber tubes 1-3 were saved for analysis