Lyophilizing Mb
Lyophilizing Mb samples for studies in organic solvents
- pH 3
- 0.0115g Mb
- 0.2005g D-sorbitol
- 0.110g (1450MW) PEG
- 10mL 10mM citrate pH 3
- pH 5
- 0.0110g Mb
- 0.1946g D-sorbitol
- 0.100g (1450MW) PEG
- 10mL 10mM acetate pH 5
- pH 7
- 0.020g Mb
- 0.1998g D-sorbitol
- 0.1085g (1450MW) PEG
- 10mL 10mM phosphate pH 7
- pH 9
- 0.0118g Mb
- 0.1847g D-sorbitol
- 0.106g (1450MW) PEG
- 10mL 10mM tris pH 9
- Take these samples and freeze them in liquid nitrogen.
- All samples are reddish brown except for the acetate, which has a greenish color.
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