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Maddie ran several PCR runs last week to try to mutate K31C Asc Hb to K31C T109C Asc Hb.
I made an agarose gel (1% - 0.25g agarose in 25mL TAE Buffer)
The lanes in the gel are
(each lane consists of 5uL DNA and 1uL loading buffer).
Lane 2 showed nothing. Lanes 3-6 were really streaky. Potentially many different products, or perhaps too concentrated. Should run again more dilute.
Last week we expressed WT Asc Mn-Hb and Ni-Hb. I am going to extract the Mn-Hb today.
Nyousha is making buffers for FPLC: 1L of 10mM Tris 50mM NaCl pH 8.5, 1L of 10mM Tris 1M NaCl pH 8.5
The buffers were filtered after they were made.