Objective
Running a fluorescence scan of AuCl3+lysine
Description
- Add experimental record here. Include what, how, and why...
Data
Stock solutions
- Lysine
- 3.93 mg lysine
- to 10.0 mL water
- 182.7 g/mol
- 2.15 mM
- AuCl3
- 32.39 mg AuCl3
- to 25.0 mL water
- 303.3 g/mol
- 4.27 mM
Sample: 0.25mM AuCl3, 137.5 μM Lysine (chosen for rough concentration of lysine in lysozyme AuNP samples), pH 4
- 300μL 1 mM HCl
- 176 μL AuCl3
- 192 μL lysine
- 2332 μL water
UV-Vis Spectra
I took UV-Vis spectra before and after the fluorescence scans.
Picture of reaction product
Fluorescence Spectra
- 290 nm excitation, 10.0 nm slit width
- Emission: 310-540 nm, 10 nm slit width
- 100 nm/min
Notes
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