User:Matt Hartings/Notebook/AU Biomaterials Design Lab/2016/09/26

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Running a fluorescence scan of AuCl3+lysine


  1. Add experimental record here. Include what, how, and why...


Stock solutions

  1. Lysine
    1. 3.93 mg lysine
    2. to 10.0 mL water
    3. 182.7 g/mol
    4. 2.15 mM
  2. AuCl3
    1. 32.39 mg AuCl3
    2. to 25.0 mL water
    3. 303.3 g/mol
    4. 4.27 mM

Sample: 0.25mM AuCl3, 137.5 μM Lysine (chosen for rough concentration of lysine in lysozyme AuNP samples), pH 4

  1. 300μL 1 mM HCl
  2. 176 μL AuCl3
  3. 192 μL lysine
  4. 2332 μL water

UV-Vis Spectra

I took UV-Vis spectra before and after the fluorescence scans.

20160926 mrh LysineAuCl3pH480C UVVis1.png

20160926 mrh LysineAuCl3pH480C UVVis2.png

Picture of reaction product

20160926 mrh lysaucl3 result.JPG

Fluorescence Spectra

  1. 290 nm excitation, 10.0 nm slit width
  2. Emission: 310-540 nm, 10 nm slit width
  3. 100 nm/min

20160926 mrh lysaucl3 IntegratedI.png

20160926 mrh lysaucl3 EmissionMax.png

20160926 mrh lysaucl3 scans1.png

20160926 mrh lysaucl3 scans2.png


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