User:Matt Hartings/Notebook/AU Biomaterials Design Lab/2016/09/26

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Running a fluorescence scan of AuCl3+lysine


  1. Add experimental record here. Include what, how, and why...


Stock solutions

  1. Lysine
    1. 3.93 mg lysine
    2. to 10.0 mL water
    3. 182.7 g/mol
    4. 2.15 mM
  2. AuCl3
    1. 32.39 mg AuCl3
    2. to 25.0 mL water
    3. 303.3 g/mol
    4. 4.27 mM

Sample: 0.25mM AuCl3, 137.5 μM Lysine (chosen for rough concentration of lysine in lysozyme AuNP samples), pH 4

  1. 300μL 1 mM HCl
  2. 176 μL AuCl3
  3. 192 μL lysine
  4. 2332 μL water

UV-Vis Spectra

I took UV-Vis spectra before and after the fluorescence scans.

Picture of reaction product

Fluorescence Spectra

  1. 290 nm excitation, 10.0 nm slit width
  2. Emission: 310-540 nm, 10 nm slit width
  3. 100 nm/min


This area is for any observations or conclusions that you would like to note.

Use categories like tags. Change the "Course" category to the one corresponding to your course. The "Miscellaneous" tag can be used for particular experiments, as instructed by your professor. Please be sure to change or delete this tag as required so that the categories remain well organized.