User:Matt Hartings/Notebook/AU Biomaterials Design Lab/2016/09/23

Biomaterials Design Lab

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Objective Take fluorescence scans of tryptophan and tryptophan+AuCl3 at 80C and pH 4. Description Add experimental record here. Include what, how, and why... Data Stock Solutions Tryptophan 3.00 mg tryptophan to 10.0 mL of water 204.23 g/mol 1.47 μM Reaction solutions Just Tryptophan (3.0 μM tryptophan pH 4) 300 μL 1 mM HCl 6.1 μL tryptophan 2694 uL water 3.0μM tryptophan, 0.25mM AuCl3, pH 4 300 μL 1 mM HCl 6.1 μL tryptophan 201 μL AuCl3 2493 uL water UV-Vis W samples I took UV-Vis spectra of the samples before and after the fluorescence scans Fluorescence W samples Fluorescence measurements 290 nm excitation, 10.0 nm slit width Emission: 310-540 nm, 10 nm slit width 100 nm/min 1 scan every 3 minutes UV-Vis W+AuCl3 samples I took UV-Vis spectra of the samples before and after the fluorescence scans Note that there is not a hint of purple color in the sample after the reaction. Fluorescence W samples Fluorescence measurements 290 nm excitation, 10.0 nm slit width Emission: 310-540 nm, 10 nm slit width 100 nm/min 1 scan every 3 minutes Note: the second to last scan didn't save properly. Notes This area is for any observations or conclusions that you would like to note. Use categories like tags. Change the "Course" category to the one corresponding to your course. The "Miscellaneous" tag can be used for particular experiments, as instructed by your professor. Please be sure to change or delete this tag as required so that the categories remain well organized.