User:Matt Hartings/Notebook/AU Biomaterials Design Lab/2016/08/31

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Objective

Samples for group A

Make samples using the stock solutions I prepped yesterday.


Stock solutions for groups B and C experiments

Make stock solutions of lysozyme and myoglobin for experiments

Description

Samples for group A

Solutions were added to glass test tubes, covered with aluminum foil, and placed in an oven (set to 80°C) for 4 hours.

Data

Stock solutions

  1. Lysozyme
    1. 8.88 mg of lysozyme with water in 10.0 mL volumetric flask
    2. MW = 14307 g/mol
    3. concentration = 62.1 μM
  2. Myoglobin
    1. 13.71 mg of myoglobin with water in 10.0 mL volumetric flask
    2. MW = 16951 g/mol
    3. concentration = 80.9 μM
  3. AuCl3
    1. 12.6 mg of AuCl3 with water in 10.0 mL volumetric flask
    2. MW = 303.3 g/mol
    3. concentration = 4.17 mM


Instructions for making samples for kinetics experiments

  1. pH 4
    1. Lysozyme: 0.25mM Au, 12.5μM Lysozyme, 3mL total
      1. 225 μL AuCl3
      2. 300 μL 1M HCl
      3. 604 μL Lysozyme
      4. 1871 μL Water
    2. Myoglobin: 0.25mM Au, 12.5μM Myoglobin, 3mL total
      1. 225 μL AuCl3
      2. 300 μL 1M HCl
      3. 464 μL Myoglobin
      4. 1871 μL Water

AuNP synthesis results

pH 4 through 12 (left to right), 0.25 mM Au, 3.1 μM BSA, 0 mM fructose

20160831 mrh BSAAuNP.JPG

pH 4 through 12 (left to right), 0.25 mM Au, 3.1 μM BSA, 0.0625 mM fructose

20160831 mrh BSAFructoseAuNP.JPG

Notes

This area is for any observations or conclusions that you would like to note.


Use categories like tags. Change the "Course" category to the one corresponding to your course. The "Miscellaneous" tag can be used for particular experiments, as instructed by your professor. Please be sure to change or delete this tag as required so that the categories remain well organized. Sarah Burkhard

Patrick Hampson

Hannah Harvey

Anneliese Faustino 160831

User:Sara K. Mattson/Notebook/Chemistry 671/2016/08/31