Objective
Cassidy had previously incubated the Fe3O4 microgels with different metal salts.
Today I am going to run ICP to determine the concentration of metal left in solution.
Description
Standards
I am using the ICP multielement standard solution VII from Millipore (1.09492.0100, Lot HC43301592)
The rough concentration for all of the metal ions in this solution are 100 mg/L
 Solution 1
 Concentration: 10 mg/L
 Volume of standard used: 5 mL
 Final Volume: 50 mL
 Solution 2
 Concentration: 5 mg/L
 Volume of standard used: 2.5 mL
 Final Volume: 50 mL
 Solution 3
 Concentration: 1 mg/L
 Volume of standard used: 0.5 mL
 Final Volume: 50 mL
 Solution 4
 Concentration: 0.75 mg/L
 Volume of standard used: 0.375 mL
 Final Volume: 50 mL
 Solution 5
 Concentration: 0.5 mg/L
 Volume of standard used: 0.25 mL
 Final Volume: 50 mL
 Solution 6
 Concentration: 0.25 mg/L
 Volume of standard used: 0.125 mL
 Final Volume: 50 mL
 Solution 7
 Concentration: 0.1 mg/L
 Volume of standard used: 0.05 mL
 Final Volume: 50 mL
Note for solutions 6 and 7 I used a P200 pipet and added all of the solution in the tip. I did this based on a volume calibration that I had done before taking making solutions.
Metal Concentration In Standards (mg/L) and wavelengths used to evaluate concentration
 Cd (214.439 nm)
 Solution 1: 10.0
 Solution 2: 5.00
 Solution 3: 1.00
 Solution 4: 0.750
 Solution 5: 0.500
 Solution 6: 0.250
 Solution 7: 0.100
 Co (230.786 and 238.892 nm)
 Solution 1: 9.90
 Solution 2: 4.95
 Solution 3: 0.990
 Solution 4: 0.743
 Solution 5: 0.495
 Solution 6: 0.248
 Solution 7: 0.0990
 Cr (267.716 nm)
 Solution 1: 9.90
 Solution 2: 4.95
 Solution 3: 0.990
 Solution 4: 0.743
 Solution 5: 0.495
 Solution 6: 0.248
 Solution 7: 0.0990
 Cu (327.395 nm)
 Solution 1: 10.0
 Solution 2: 5.00
 Solution 3: 1.00
 Solution 4: 0.750
 Solution 5: 0.500
 Solution 6: 0.250
 Solution 7: 0.100
 Fe (238.204 and 259.940 nm)
 Solution 1: 10.1
 Solution 2: 5.05
 Solution 3: 1.01
 Solution 4: 0.758
 Solution 5: 0.505
 Solution 6: 0.253
 Solution 7: 0.101
 K (766.491 nm)
 Solution 1: 9.8
 Solution 2: 4.90
 Solution 3: 0.98
 Solution 4: 0.735
 Solution 5: 0.490
 Solution 6: 0.245
 Solution 7: 0.0980
 Mg (279.553 nm)
 Solution 1: 10.0
 Solution 2: 5.00
 Solution 3: 1.00
 Solution 4: 0.750
 Solution 5: 0.500
 Solution 6: 0.250
 Solution 7: 0.100
 Mn (257.610 nm)
 Solution 1: 10.0
 Solution 2: 5.00
 Solution 3: 1.00
 Solution 4: 0.750
 Solution 5: 0.500
 Solution 6: 0.250
 Solution 7: 0.100
 Na (588.995 nm)
 Solution 1: 10.1
 Solution 2: 5.05
 Solution 3: 1.01
 Solution 4: 0.758
 Solution 5: 0.505
 Solution 6: 0.253
 Solution 7: 0.101
 Ni (227.021 and 230.299 nm)
 Solution 1: 10.0
 Solution 2: 5.00
 Solution 3: 1.00
 Solution 4: 0.750
 Solution 5: 0.500
 Solution 6: 0.250
 Solution 7: 0.100
 Pb (220.353 nm)
 Solution 1: 10.0
 Solution 2: 5.00
 Solution 3: 1.00
 Solution 4: 0.750
 Solution 5: 0.500
 Solution 6: 0.250
 Solution 7: 0.100
 Zn (213.857 nm)
 Solution 1: 9.90
 Solution 2: 4.95
 Solution 3: 0.990
 Solution 4: 0.743
 Solution 5: 0.495
 Solution 6: 0.248
 Solution 7: 0.0990
 Note: These wavelengths were chosen so that there was minimal overlap with the other wavelengths being used and that maximized the signal from the element of interest.
Running Conditions Agilent Technologies 5100 ICPOES
 Replicates 3
 Pump Speed: 12 rpm
 Sample Introduction: Manual
 Uptake delay: 5s
 Read Time: 5s
 RF power: 1.20 kW
 Stabilization time: 5s
 Viewing mode: Axial
 Viewing Height: 8mm
 Nebulizer Flow: 0.7 L/min
 Plasma Flow: 12 L/min
 Aux flow: 1 L/min
 Make up flow: 0 L/min
Sample Names
 hyd FeNP Pb acetate 3.79mg/L
 dry FeNP Pb acetate 3.79mg/L
 Pb acetate 3.79mg/L
 dry FeNP Cd acetate 1.12mg/L
 hyd FeNP Cd acetate 1.12mg/L
 Cd acetate 1.12mg/L
 hyd FeNP Mg SO4 1.2mg/L
 dry FeNP Mg SO4 1.2mg/L
 Mg SO4 1.2mg/L
 hyd FeNP Cr Cl3 2.66mg/L
 dry FeNP Cr Cl3 2.66mg/L
 Cr Cl3 2.66mg/L pulled too soon … rerunning
 Cr Cl3 2.66mg/L
 hyd FeNP Zn SO4 2.88mg/L
 dry FeNP Zn SO4 2.88mg/L
 Zn SO4 2.88mg/L
 hyd FeNP Ni SO4 2.63mg/L
 dry FeNP Ni SO4 2.63mg/L
 Ni SO4 2.63mg/L
 hyd FeNP Co Cl2 2.38mg/L
 dry FeNP Co Cl2 2.38mg/L
 Co Cl2 2.38mg/L
 hyd FeNP Mn Cl2 1.98mg/L
 dry FeNP Mn Cl2 1.98mg/L
 Mn Cl2 1.98mg/L
 hyd FeNP Cu Cl2 1.705mg/L
 dry FeNP Cu Cl2 1.705mg/L
 Cu Cl2 1.705mg/L
Calibration Curves
Not all standards gave good data for the calibration curves. Some concentrations were too high. The following points were used:
 Cd (214.439 nm)
 Solution 2: 5.00
 Solution 3: 1.00
 Solution 4: 0.750
 Solution 5: 0.500
 Solution 6: 0.250
 Solution 7: 0.100
 Co (230.786 and 238.892 nm)
 Solution 2: 4.95
 Solution 3: 0.990
 Solution 4: 0.743
 Solution 5: 0.495
 Solution 6: 0.248
 Solution 7: 0.0990
 Cr (267.716 nm)
 Solution 2: 4.95
 Solution 3: 0.990
 Solution 4: 0.743
 Solution 5: 0.495
 Solution 6: 0.248
 Solution 7: 0.0990
 Cu (327.395 nm)
 Solution 1: 10.0
 Solution 2: 5.00
 Solution 3: 1.00
 Solution 4: 0.750
 Solution 5: 0.500
 Solution 6: 0.250
 Solution 7: 0.100
 Fe (238.204 and 259.940 nm)
 Solution 2: 5.05
 Solution 3: 1.01
 Solution 4: 0.758
 Solution 5: 0.505
 Solution 6: 0.253
 Solution 7: 0.101
 K (766.491 nm) *Special Circumstances more info below
 Solution 1: 9.8
 Solution 2: 4.90
 Solution 3: 0.98
 Solution 4: 0.735
 Solution 5: 0.490
 Solution 6: 0.245
 Solution 7: 0.0980
 Mg (279.553 nm)
 Solution 3: 1.00
 Solution 4: 0.750
 Solution 5: 0.500
 Solution 6: 0.250
 Solution 7: 0.100
 Mn (257.610 nm)
 Solution 3: 1.00
 Solution 4: 0.750
 Solution 5: 0.500
 Solution 6: 0.250
 Solution 7: 0.100
 Na (588.995 nm) *Special Circumstances more info below
 Solution 1: 10.1
 Solution 2: 5.05
 Solution 3: 1.01
 Solution 4: 0.758
 Solution 5: 0.505
 Solution 6: 0.253
 Solution 7: 0.101
 Ni (227.021 and 230.299 nm)
 Solution 2: 5.00
 Solution 3: 1.00
 Solution 4: 0.750
 Solution 5: 0.500
 Solution 6: 0.250
 Solution 7: 0.100
 Pb (220.353 nm)
 Solution 1: 10.0
 Solution 2: 5.00
 Solution 3: 1.00
 Solution 4: 0.750
 Solution 5: 0.500
 Solution 6: 0.250
 Solution 7: 0.100
 Zn (213.857 nm)
 Solution 3: 0.990
 Solution 4: 0.743
 Solution 5: 0.495
 Solution 6: 0.248
 Solution 7: 0.0990
 For low values of K, the following standards were used
 Solution 4: 0.735
 Solution 5: 0.490
 Solution 6: 0.245
For these points the calibration curve is:
Intensity = 81938.54*Concentration + 3311.66
 For high values of K, the following standards were used
 Solution 2: 4.90
For these points the calibration curve is:
Intensity = 107499.38*Concentration + 3336.50
All of the data for Na was at/near/or below the baseline value. All Na values are assumed to be zero. Which they should be ... there was no Na used anywhere in this study.
Data
 Add data and results here...
Notes
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