User:Matt Hartings/Notebook/AU Biomaterials Design Lab/2015/09/15

Biomaterials Design Lab

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Objective Today we're going to make more fibers for analysis and use the rest of the semester. We'll be using the protocols we used on September 2nd. The only difference is that we'll use the stock concentrations that I made today (see below). I want to complete the analysis of the UV-Vis data. We need to compile a master sheet with everyone's absorbance vs concentration data in order to make a composite determination of the molar absorptivity of lysozyme I want a master spread sheet and data analysis of eppendorf tube masses. Each group needs to have measured 15-20 tubes. I also want everyone working on their writing assignment. I'll help you out with that during the class. Description Target Concentrations Based on the total reaction volume that the class will be using and the final concentrations that will be in each reaction volume, I have determined that the following HAuCl4·H2O and Lysozyme masses are appropriate for today's stock solutions. Stock solutions HAuCl4·H2O 2.5 mM MW = 339.79 g/mol Volume = 50 mL 42.4 mg Lysozyme 20 μM MW = 14307 g/mol Volume = 100 mL 28.6 mg Actual Concentrations The actual masses are different from the target masses. The concentrations shown below reflect the actual concentrations made for the stock solutions. I used a 50 mL volumetric flask to make the gold stock solution and a 100 mL volumetric flask to make the protein solution. In each case I used HPLC-grade water. Stock solutions HAuCl4·H2O 50.38 mg MW = 339.79 g/mol Volume = 50 mL 2.96 mM Lysozyme 28.69 mg MW = 14307 g/mol Volume = 100 mL 20.1 μM Directions for making samples Note: The final concentration of gold should be 0.25 mM and the final concentration of lysozyme should be 5.556 μM. This gives a Au:lysozyme ratio of 45, which should yield fibers. 1 mL samples Volume of gold stock: 84.4 μL Volume of protein stock: 276 μL Volume of water: 640 μL 5 mL samples Volume of gold stock: 422 μL Volume of protein stock: 1,380 μL Volume of water: 3,198 μL Data Add data and results here... Notes This area is for any observations or conclusions that you would like to note. Use categories like tags. Change the "Course" category to the one corresponding to your course. The "Miscellaneous" tag can be used for particular experiments, as instructed by your professor. Please be sure to change or delete this tag as required so that the categories remain well organized.