User:Matt Hartings/Notebook/AU Biomaterials Design Lab/2012/05/23

Biomaterials Design Lab

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Objective Prepping samples to bring to NIST for dynamic light scattering measurements tomorrow. Description I made 2 solutions (11.8 mg of HAuCl4·H2O in 14 mL of water and 23.2 mg of BSA in 22mL of water). I then proceeded to use these solutions to prepare my samples for tomorrow. } Data Something strange happened in the notes section ... Here is the data: I made 2 solutions (11.8 mg of HAuCl4 in 14 mL of water and 23.2 mg of Bovine Serum Albumin in 22 mL of water) and used these solutions to make samples for preparing AuNPs. Approx. Ratio Au/BSA Volume Au added (mL) Volume BSA added (mL) Volume Water added (mL) 20 0.50 4.167 0.333 30 0.50 2.777 1.723 40 0.50 2.083 2.417 50 0.50 1.667 2.833 60 0.50 1.389 3.111 70 0.50 1.190 3.310 80 0.50 1.042 3.458 90 0.50 0.927 3.573 100 0.50 0.833 3.667 110 0.50 0.757 3.743 120 0.50 0.693 3.807 130 0.50 0.640 3.860 140 0.50 0.594 3.906 150 0.50 0.556 3.944 160 0.50 0.520 3.980 170 0.50 0.49 4.010 } These samples were placed in the oven at 80°C for 4 hours. After 3 hours, this is what the solutions look like Here is the UVVis Data part 1 Here is the UVVis Data part 2 Notes This area is for any observations or conclusions that you would like to note. Use categories like tags. Change the "Course" category to the one corresponding to your course. The "Miscellaneous" tag can be used for particular experiments, as instructed by your professor. Please be sure to change or delete this tag as required so that the categories remain well organized. Approx. Au/BSA ratio Volume Au added (mL) Volume BSA added (mL) Volume Water added (mL) Actual Ratio 20 0.50 4.167 0.333 18.8 30 0.50 2.777 1.723 28.3 40 0.50 2.083 2.417 37.7 50 0.50 1.667 2.833 47.1 60 0.50 1.389 3.111 56.5 70 0.50 1.190 3.310 66.0 80 0.50 1.042 3.458 75.3 90 0.50 0.927 3.573 84.7 100 0.50 0.833 3.667 94.2 110 0.50 0.757 3.743 103.7 120 0.50 0.693 3.807 113.2 130 0.50 0.640 3.860 122.6 140 0.50 0.594 3.906 132.1 150 0.50 0.556 3.944 141.2 160 0.50 0.520 3.980 150.9 170 0.50 0.490 4.010 160.2