In the original protocol, the instruction called for vacuum filtration of our mixture, tributylhexadecylphosphonium bromide, through a 0.2μm nylon fiber.
A consensual decision was made that placing the mixtures in centrifuge would provide better results of separation.
The sample weighed the amount of 17.69 g. Addition of water was made to balance the viscous mixture, (1-hexadecyl)triphenylphosphonium bromide), from Eleni's group. The final weight was 27.42 g.
The mixture was centrifuged for 30 minutes at 4000 rpm, 4°C. The centrifuge used was the make of Thermo Scientific Sorvall RC 6+ Centrifufge.
The rotor chosen to perform the centrifuge was SH-3000 4700 max.
At 10:53 AM, samples were observed to have three distinct layers; top and bottom layer were clay, and the middle was composed of water/supernatant.
The supernatant was collected and transferred into a 50 mL falcon tube. This was stored if additional analysis was needed in the future.
Two sets of washes are required for the exchanged clay. Each wash comprised of washing the clay with water to remove adsorbed NaCl and ethanol to remove remaining surfactants.
After the washes were completed by Dr. Hartings, the mixture was stored in an oven to evaporate excess water.
Powder X-RAY Diffraction of MG-PVA-clay samples
Eleni Kalivas executed the introduction of the usage of the powder X-RAY diffraction instrument. The make of the instrument is by Rigaku MiniFlex II.
The parameters set for the data collection made via the Standard Measurement interface are as follows:
2° start angle
40° stop angle
0.5 inch sampling width
1°/minute scanning speed
The original protocol stated a 1°/second scanning speed
Our group provided the blank PVA film which was placed in position 4. Other groups' PVA films were placed in the following order:
Position 1: Dr. Hartings' solid, sodium montmorillonite
Position 2: 8 ppm MG from Madeleine and Alicia
Position 3: group's 2 ppm MG
Position 5: 80 ppm MG from Melvin, Eleni, and Becca
Position 6: 200 ppm MG from Andrew, Michael, and Tami
Project name
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