User:Mariana Ruiz Velasco L./Notebook/IGEM 2010/Wet lab journal/2010/10/11

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Manzano's day :D


  • Today, I ran a gel to check if the ligations were done correctly.




  • The lanes were as follows, everything restricted with ECO RI and PST I:


1,2 . Mutated red luciferase.

3. LRE ligated into pSB1C3.

4. LRE ligated into BBa_J61002.

5. EPIC with LRE.

6. L. cruciata with LRE.