User:Mariana Ruiz Velasco L./Notebook/IGEM 2010/Wet lab journal/2010/10/10

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Come on and do it!


  • Today, I extracted plasmid from several colonies of EPIC + LRE, L. cruciata + LRE, two colonies of the mutated luciferase, LRE with J23100, and LRE in pSB1C3.


  • I left a restriction with ECO RI and PST I for a total of 20μl to check if everything was ok and I left it incubating at 37°C ON :D