User:Mariana Ruiz Velasco L./Notebook/IGEM 2010/Wet lab journal/2010/09/30

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There we go again


  • I came to the lab to do a transformation of my ligations. The plates are as follows:


1. Ligation of J23100 with mutated red luciferase in ampicilin 2-O.

2. Ligation of J23100 with mutated red luciferase in ampicilin 2-P.

3. Ligation of J23106 with mutated red luciferase in ampicilin 2-O.

4. Positive control (purified plasmid of LRE with pSB1C3 in chloramphenicol.

5. Control of competent cells in LB.

  • Cultures were left ON at 37°C.