User:Mariana Ruiz Velasco L./Notebook/IGEM 2010/Wet lab journal/2010/09/29

From OpenWetWare
Jump to: navigation, search
Logo light minimal full res.jpg WiFi coli: A communicolight system Report.pngMain project page
Resultset previous.pngPrevious entry      Next entryResultset next.png

Trying to handle as many things as possible!!!


  • Today, I checked the gel of my restrictions and as I observed the correct size in a reasonable concentration, I ligated them using the following reaction for a total of 20μl:


-H2O --------------------> 7μL

-Buffer ligase ----------> 4μL

-Vector (plasmid) -----> 2μL

-Insert (luciferase) ---> 6μL

-Ligase -----------------> 1μL

  • I left the ligations at 16°C ON.