User:Mariana Ruiz Velasco L./Notebook/IGEM 2010/Wet lab journal/2010/06/10

From OpenWetWare
Jump to: navigation, search
Logo light minimal full res.jpg WiFi coli: A communicolight system Report.pngMain project page
Resultset previous.pngPrevious entry      Next entryResultset next.png

Checking on ligation

  • Today, after inactivating the restriction enzymes, I proved via electrophoresis that the ligation was done correctly.

Luc et TT.JPG

  • The lanes are:

1. Ladder
2 and 3. purified plasmid
4. Restriction with ECO RI and PST I for the purified plasmid.
5. Purified PCR product of luciferase (also the positive control).
6. Negative control.