User:Mariana Ruiz Velasco L./Notebook/IGEM 2010/Wet lab journal/2010/06/10

WiFi coli: A communicolight system	Main project page Previous entry      Next entry
Checking on ligation Today, after inactivating the restriction enzymes, I proved via electrophoresis that the ligation was done correctly. The lanes are: 1. Ladder 2 and 3. purified plasmid 4. Restriction with ECO RI and PST I for the purified plasmid. 5. Purified PCR product of luciferase (also the positive control). 6. Negative control.