User:Mariana Ruiz Velasco L./Notebook/IGEM 2010/Wet lab journal/2010/06/10

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Checking on ligation


  • Today, after inactivating the restriction enzymes, I proved via electrophoresis that the ligation was done correctly.


Luc et TT.JPG


  • The lanes are:

1. Ladder
2 and 3. purified plasmid
4. Restriction with ECO RI and PST I for the purified plasmid.
5. Purified PCR product of luciferase (also the positive control).
6. Negative control.