Woohoo!!! Transformed strain is a win!!!
- When I arrived in the morning, I saw that the strains that I plated yesterday grew well, so I inoculated them in 4mL of liquid LB medium with 4μL of tetracycline. Incubation for 7 hours at 37°C.
- Afterwards, I extracted plasmid by using the Roche High Pure Plasmid Isolation Kit. The tubes are labeled as: plasmido purif. lucif + TT Mariana 9/06/10 (1-G).
- Finally, I did a restriction for a total of 20μL as follows and labeled it as Restriccion ECO RI y PST Mariana (1-H):
-H2O -------> 10μl
-Buffer 2 --> 2μl
-BSA ------> 1μl
-DNA -------> 5μl
-ECOR1 -----> 1μl
-PST1 ------> 1μl
- Restrictions were incubated at 37°C ON(overnight).