User:Madeleine Y. Bee/Notebook/Single Molecule Fluorescence/2013/11/13

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November 13, 2013


  • Synthesis of ZnPPIX
    • 1:1 molar ratio of Zinc nitrate hexahydrate to Dr Miller's porphyrin
    • Dissolve 0.0054g Zn nitrate and 0.0144g porphyrin in 5mL DMF
    • Take absorbance measurements every 20 minutes, making 100x dilutions
    • Continue heating until absorbance spectra stops changing (80 minutes)


2013 1113 abs ZnPPIX.PNG<br.> Beer's Law calculations: 2013 1113 ZnPPIX beer's law calcs.PNG


  • In the third, fourth, and fifth samples, it is evident that the spectra stopped changing and the reaction was assumed to be complete