User:Madeleine Y. Bee/Notebook/Single Molecule Fluorescence/2013/11/13

From OpenWetWare
Jump to: navigation, search
Owwnotebook icon.png Project name <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>      </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>

November 13, 2013

Procedures

  • Synthesis of ZnPPIX
    • 1:1 molar ratio of Zinc nitrate hexahydrate to Dr Miller's porphyrin
    • Dissolve 0.0054g Zn nitrate and 0.0144g porphyrin in 5mL DMF
    • Take absorbance measurements every 20 minutes, making 100x dilutions
    • Continue heating until absorbance spectra stops changing (80 minutes)

Data

2013 1113 abs ZnPPIX.PNG<br.> Beer's Law calculations: 2013 1113 ZnPPIX beer's law calcs.PNG

Notes

  • In the third, fourth, and fifth samples, it is evident that the spectra stopped changing and the reaction was assumed to be complete