User:Madeleine Y. Bee/Notebook/Single Molecule Fluorescence/2013/10/23

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October 23, 2013

Happy Mole Day!


  • Dissolve filtered ZnPPIX from 10/16/2013 in DMF
  • Prepare several dilutions
  • Run absorbance measurements
  • Use Beer's law to determine concentration


  • 0.0026g ZnPPIX dissolved in 7mL DMF
  • MW ZnPPIX: 626.032g/mol
  • (0.0026g)×(1/626.032g/mol)×(1/0.007L)≈0.5933mM
    • assuming entire sample came off weigh boat
  • Beer's Law calculations for absorbance measurements determined the concentration to be ≈0.08mM
    • 2013 1023 ZnPPIX beers law calcs.PNG

Absorbance Spectra and Literature Comparison:

2013 1023 ZnPPIX abs spectra (mine).PNG<br.> 2013 1023 ZnPPIX abs spectra.PNG<br.> from "Metal and redox selectivity of protoporphyrin binding to the heme chaperone CcmE"