User:Madeleine Y. Bee/Notebook/Single Molecule Fluorescence/2013/10/23

ZnPPIX Sample Prep

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October 23, 2013 Happy Mole Day! Procedures Dissolve filtered ZnPPIX from 10/16/2013 in DMF Prepare several dilutions Run absorbance measurements Use Beer's law to determine concentration Data/Calculations 0.0026g ZnPPIX dissolved in 7mL DMF MW ZnPPIX: 626.032g/mol (0.0026g)×(1/626.032g/mol)×(1/0.007L)≈0.5933mM assuming entire sample came off weigh boat Beer's Law calculations for absorbance measurements determined the concentration to be ≈0.08mM Absorbance Spectra and Literature Comparison: <br.> <br.> from "Metal and redox selectivity of protoporphyrin binding to the heme chaperone CcmE"