User:Madeleine Y. Bee/Notebook/Single Molecule Fluorescence/2013/10/02

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October 2, 2013 Procedures Absorbance measurements and Beer's law calculations for samples from 10/25/2013 Sample preparation based on calculated concentrations Data Absorbance Measurements from 10/25/2013 <br.> Beer's Law calculations:<br.> Absorbance Measurements, Today I decided to run two more samples at higher concentrations on the UV-vis and calculate the concentrations from these because I needed higher concentrations in order to make my samples at the concentrations I've been using. <br.> <br.> Beer's Law calculations:<br.> Samples Prepared "20uM" and "50uM" ZnPPIX dilutions made today, fresh aliquots of all three DNA strands, and 23.5nM AuNps were used to prepare these samples 2.47uM ZnPPIX 2.47uM ZnPPIX/1.11uM DNA-SH 2.47uM ZnPPIX/1.11uM DNA (4) 2.47uM ZnPPIX/1.11uM DNA (8) 1.934uM ZnPPIX/0.94uM DNA-SH/11.75nM AuNPs 1.934uM ZnPPIX/0.74uM DNA (4)/11.75nM AuNPs 1.934uM ZnPPIX/0.74uM DNA (8)/11.75nM AuNPs 2.47uM ZnPPIX/11.75nM AuNPs Notes Run today's samples for absorbance and fluorescence Ask Dr Hartings for more ZnPPIX