User:Madeleine Y. Bee/Notebook/Single Molecule Fluorescence/2013/09/25

Sample Preparation

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September 25, 2013 Notes Since I have been unable to replicate the procedure done previously in which I observed signal with the ZnPPIX, there are a couple of procedures I want to attempt in order to identify the problem:<br.> ZnPPIX absorbance spectra to find the molar absorptivity, ε Check concentration (as well as DNA) Get new DNA samples, parse them out to smaller aliquots Switch to 4 and 8 strand DNA, adjusting [ZnPPIX] as needed Ask Dr Hartings about ZnPPIX storage/time? Look at Ellen/Eleni's ZnPPIX absorbance spectrum Check DNA with ThT Procedures Parse out 53.1uM DNA into 10uL aliquots to prevent as much freeze/thaw of overall sample as possible Take UV-vis measurements of 5mM ZnPPIX 10uM ZnPPIX 5uM ZnPPIX 100x diluted porphyrin with Zn(NO3)2·6H2O