User:Madeleine Y. Bee/Notebook/Single Molecule Fluorescence/2013/09/25

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September 25, 2013

Notes

Since I have been unable to replicate the procedure done previously in which I observed signal with the ZnPPIX, there are a couple of procedures I want to attempt in order to identify the problem:<br.>

  • ZnPPIX absorbance spectra to find the molar absorptivity, ε
    • Check concentration (as well as DNA)
  • Get new DNA samples, parse them out to smaller aliquots
    • Switch to 4 and 8 strand DNA, adjusting [ZnPPIX] as needed
  • Ask Dr Hartings about ZnPPIX storage/time?
  • Look at Ellen/Eleni's ZnPPIX absorbance spectrum
  • Check DNA with ThT

Procedures

  1. Parse out 53.1uM DNA into 10uL aliquots to prevent as much freeze/thaw of overall sample as possible
  2. Take UV-vis measurements of
    • 5mM ZnPPIX
    • 10uM ZnPPIX
    • 5uM ZnPPIX
    • 100x diluted porphyrin with Zn(NO3)2·6H2O