July 23, 2013
Procedure:Silica-coating AuNPs by MHA, Day Two Round 2
- Day Two:
- Prepare the following solutions:
- 1mL 9.4nM, 9.65nm AuNP&MHA, isopropanol/4μL TES/0.125mL NH4OH/5mL propanol
- 1mL 9.4nM, 9.65nm AuNP&MHA, isopropanol/3μL TES/0.125mL NH4OH/5mL propanol
- 1mL 9.4nM, 9.65nm AuNP&MHA, isopropanol/2μL TES/0.125mL NH4OH/5mL propanol
- 1mL 9.4nM, 9.65nm AuNP&MHA, isopropanol/1μL TES/0.125mL NH4OH/5mL propanol
- 1mL 9.4nM, 9.65nm AuNP&MHA, PBS/4μL TES/0.125mL NH4OH/5mL propanol
- 1mL 9.4nM, 9.65nm AuNP&MHA, PBS/3μL TES/0.125mL NH4OH/5mL propanol
- 1mL 9.4nM, 9.65nm AuNP&MHA, PBS/2μL TES/0.125mL NH4OH/5mL propanol
- 1mL 9.4nM, 9.65nm AuNP&MHA, PBS/1μL TES/0.125mL NH4OH/5mL propanol
- Stir solutions for one hour to allow reaction to proceed
- Centrifuge solutions at 4000 rpm for one hour
- Re-suspend particles in 140uL PBS (per solution)
- Dilute each solution 10 times
Notes from Meeting
- Don't compare absorbance spectra
- Peak shift > color change > aggregation indication?
- Time for silica-coating procedure?
- Could we use larger AuNP@SiO2? since we know we can make them
- Igor Pro: SiO2 thickness
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