User:Madeleine Y. Bee/Notebook/Single Molecule Fluorescence/2013/07/24
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July 24, 2013
Procedure:Silica-coating AuNPs by MHA, Day Two Round 3
Notes and Observations
MHA and AuNP solution was heated just enough to allow MHA to dissolve. 2uL and 4uL samples appeared much clearer than other samples which had visible particles floating around in solution (prior to centrifugation). It looked as if the nanoparticles had stuck to the bottom of these two vials. After centrifugation, 2uL and 4uL samples produced noticeably less particles and were visibly a lighter color than the other samples. All samples seemed to be more purple in color than the pink characteristic of gold nanoparticles. This may be similar to what happened with the samples we made and took TEM images of last week, and could be indicative of aggregation.
For NIST Tomorrow