User:Madeleine Y. Bee/Notebook/Single Molecule Fluorescence/2013/07/05
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July 5, 2013FCS Sample Prep
FCS DataGraphs generated using Igor Pro.<br.> <br.> <br.> 10000x diluted green fluorescent beads were used to align the laser. Sample was run three times, for 90s each.<br.> <br.> <br.> PBS run to check for background fluorescence. Sample was run three times, 200s each to eliminate noise.<br.> <br.> <br.> <br.> <br.> <br.> <br.> <br.> <br.> <br.> All DNA/MB samples run three times, for 500s.<br.> <br.> Calibration Graph:<br.>
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