User:Madeleine Y. Bee/Notebook/Single Molecule Fluorescence/2013/07/03
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July 3, 2013FCS Sample Prep
FCS Data
NotesThe inconsistency of the 50pM DNA/40pM MB sample has been observed twice in a row, which may indicate a lower detection limit around this concentration. However, since both sets of samples that have shown this inconsistency were made from the same dilutions (made on 07/02/2013), new samples will be completely remade to see if this trend continues. To look into this possibility further and for the construction of a calibration graph, 25pM DNA/20pM MB and 125pM DNA/100pM MB samples will be made for the next run.<br.> PBS will also be run again, multiple times, for 200-300s, as the fluorescence and noise observed was significantly higher than expected. All samples have been diluted with PBS. <br.> Green fluorescent beads are always run first to align laser for all following samples.<br.>
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