User:Madeleine Y. Bee/Notebook/Single Molecule Fluorescence/2013/06/24

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June 24, 2013

DNA/ThT Samples Dilutions

Updated fluorescence data and calibration curve for DNA/ThT solutions.<br.> Fluor data 2013 0624 varying DNA-ThT.png<br.> <br.> Fluor data 2013 0624 varying DNA-ThT calibration curve.png<br.> <br.>


The 700nM sample has been made several different times and has yielded inconsistent results. The sample may be remade again because I feel that the results will significantly change the equation of the calibration curve. With the current data, the 800nM appears to be an outlier whereas if an accurate fluorescence reading could be produced from the 700nM sample, the equation would probably be far more accurate.