September 24, 2014
Procedures
Completing Film Synthesis from Last Week
- Warm 150mL of Sodium Sulfate Solution (see below for details) at 80C for 15 minutes
- Place film in Sodium Sulfate Solution (see below for details) at 80C for 1 hour with gentle stirring
- Warm 150mL of 1% HCl at 80C for 15 minutes
- Place film in 1% HCl Solution (see below for details) at 80C for 30 minutes with gentle stirring
- Place film in Sodium Bicarbonate Solution for 15 minutes at room temperature with gentle stirring
Make 10% Exchanged Clay Films
- From our exchanged clay
- Add 7mL of water to 1.0028 g of 22,000MW polyvinyl alcohol and 0.1107 g of exchanged NaMT in a glass vial
- Stir the solution with constant heating at 75C until the polymer is dissolved and the clay is dispersed (~15 minutes)
- Cool the solution to room temperature
- Add 0.5mL 0.8 wt% glutaraldehyde.
- Stir for an additional 3 minutes to ensure homogeneity
- Pour solution into a teflon dish to dry until the next class
- From MJJ's group exchanged clay
- Add 7mL of water to 1.0042 g of 22,000MW polyvinyl alcohol and 0.1101 g of exchanged NaMT (same ion as our group) in a glass vial
- Stir the solution with constant heating at 75C until the polymer is dissolved and the clay is dispersed (~15 minutes)
- Cool the solution to room temperature
- Add 0.5mL 0.8 wt% glutaraldehyde.
- Stir for an additional 3 minutes to ensure homogeneity
- Pour solution into a teflon dish to dry until the next class
Atomic Absorption: Arsenic Solutions
- Standard solutions:
- 0ppm
- 10ppm
- 20ppm
- 30ppm
- 40ppm
- 50 ppm
- Note: all standard solutions made by James and Eleni
- Sample dilutions (up to 10mL):
- 150 ppm solutions
- 5x dilution (~30 ppm): (1x)(V)=(1/5x)(10mL)
- 100 ppm solutions
- 4x dilution (~20 ppm): (1x)(V)=(1/4x)(10mL)
- 50 ppm solutions
- No dilution
- Note: AA detector broken, this is will be continued at a later date
Soaking Films in Rhodamine 6G Dye
Film
|
Solution
|
Vial mass
|
Vial + film mass
|
Film mass
|
PVA film |
20 uM R6G |
13.0803 g |
13.1813 g |
0.101 g
|
|
10 uM R6G |
13.0713 g |
13.1497 g |
0.0784 g
|
|
1 uM R6G |
13.0403 g |
13.1337 g |
0.0934 g
|
PVA-Clay film |
20 uM R6G |
13.0828 g |
13.2554 g |
0.1726 g
|
|
10 uM R6G |
12.9552 g |
13.0732 g |
0.118 g
|
|
1 uM R6G |
13.0198 g |
13.1377 g |
0.1179 g
|
- Dilutions done by James and Eleni
- Each film soaked in 10 mL R6G solution
|