November 12, 2013
Objective
- Atomic Absorption of Lysozyme and Hemoglobin nanoparticles synthesized 11/06/2013
- UV-vis measurements of Lysozyme and Hemoglobin nanoparticles synthesized 11/06/2013
Description
Atomic Absorption:<br.>
- Centrifuge 4mL from each sample for 30min at 4000rpm
- Make standard solutions in the same manner as the last time we performed AA; this time go up to 50ug/mL
- Take measurements of your standards and samples
- Make a graph showing concentration of Au vs Au:protein ratio
Gold Standards- Calculations
- 50 ug/mL:
- (1000ug/mL)V=(50ug/mL)(5mL)
- V= 0.25mL Au stock in 4.75mL water
- Each sample diluted from Au stock in the above manner
Data
Atomic Absorption<br.>
Notes
- UV-vis measurements to be taken tomorrow
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