User:Kristina M Holton/Notebook 2007 7 3
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DNA MaxiPrep C6-C10
Someone turned off shaker in warm room -> cell culture not as turbid as it should be
Checked overnight culture's OD600 at 9:45 -> .369 (need at least .5, .6-.7 desirable)
P1 Buffer: need to bring stock Tris-HCl pH 7.0 up to 8.0; need RNase A
Cells spun down ~ 4:30, stored in 4C overnight
miRNA Extraction and Purification Using 2nd Protocol
Patient: DR
2ml might not be enough QIAzol for tissue grinder -> increased to about 3ml
Did not need first centrifuge step
Good yield RNA (~24ug)