User:Khyra A. Neal/Notebook/Experimental Biological Chemistry II Notebook/2015/03/24

Project name

Main project page Previous entry      Next entry

March 24, 2015 Objective Stock Solution Prep Prepare New Citrate Nanoparticles Prepare New Reaction Description Stock Solutions Ideally the concentration of the nanoparticles needs to be increased from 20.8 nM to 20.8 mM. In order to increase the concentration, the diameter of the nanoparticles needs to be increased. Increasing the initial concentration of HAuCl4, NaBH44, and Sodium Citrate will hopefully allow for an increase in nanoparticles 4% wt HAuCl4 = 0.4 g HAuCl4 in 10 mL H2O 38.8 mM Sodium Citrate = 0.11728 g Sodium Citrate in 10 mL Ho 0.3% wt NaBH4 = 0.012 g NaBH4 in 5 mL 38.8 mM Sodium Citrate Citrate Nanoparticles 2 mL 4% wt HAuCl4 4 mL 38.8 mM Sodium Citrate 0.3% wt NaBH4 The concentration of the HAuCl4 was too high causing the gold to clump in solution. Another batch was made by just doubling the original amounts added based on March 17, 2015 90 mL H2O to 100 mL volumetric flask Add 2 mL 1% wt HAuCl4 and stir for 1 minute Add 4 mL 38.8 mM Sodium Citrate and stir for 1 minute Add 2 mL 0.075% wt. NaBH4 and stir for 5 minutes The solution turned a deep burgundy/purple after the addition of 2 mL 0.075% NaBH4 New Reaction A new reaction was setup as described on February 10,2015. 2 mmol 30% H2O2 was added instead of 1 mmol 2 mL Citrate AuNPs added