User:Kathryn Muratore/Notebook/AU CHEM-570 lab prep/2011/08/08

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Objective

Redo 2-stage QuikChange procedure with pMXB10 and +His primers Transform ligation reactions from last week


Bench work

  1. QuikChange 2-stage protocol with 5 cycles in the first stage instead of 1
    • 8 minute elongation step
  2. Transformed 5μL of each ligation reaction into 200μL competent DH10B and 800μL SOC