User:Kathryn Muratore/Notebook/AU CHEM-570 lab prep/2011/08/05

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Objective

Run DNA gel with ligations from last night

Gel

  • Lane 1: Ladder
  • Lane 2: pTXB1 midiprep from 7/28
  • Lane 3: pTXB1 dd re-clean 8/4
  • Lane 4: pTXB1 dd re-clean; ligated with 'Old' buffer 8/4
  • Lane 5: pTXB1 dd re-clean; ligated with 'New' buffer 8/4
  • Lane 6: pKK223-3 BamHI digest re-clean 8/4
  • Lane 7: pKK223-3 BamHI digest re-clean; ligated with 'Old' Buffer 8/4
  • Lane 8: pKK223-3 BamHI digest re-clean; ligated with 'New' Buffer 8/4
  • Lane 9: pKK223-3 miniprep from 7/26/11 by Kay Wang
  • Lane 10: ----
  • Lane 11: pTXB1.His double digest ligation with BSA insert from yesterday
  • Lane 12: pTXB1.His double digest ligation negative control from yesterday
  • Lane 13: pTXB1 double digest ligation with BSA insert from yesterday
  • Lane 14: pTXB1 double digest ligation negative control from yesterday

Results

Dnagel8.05.2011labeled.jpg