User:Kathryn Muratore/Notebook/AU CHEM-570 lab prep/2011/08/05

AU CHEM-570 Lab Prep

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Objective Run DNA gel with ligations from last night Gel Lane 1: Ladder Lane 2: pTXB1 midiprep from 7/28 Lane 3: pTXB1 dd re-clean 8/4 Lane 4: pTXB1 dd re-clean; ligated with 'Old' buffer 8/4 Lane 5: pTXB1 dd re-clean; ligated with 'New' buffer 8/4 Lane 6: pKK223-3 BamHI digest re-clean 8/4 Lane 7: pKK223-3 BamHI digest re-clean; ligated with 'Old' Buffer 8/4 Lane 8: pKK223-3 BamHI digest re-clean; ligated with 'New' Buffer 8/4 Lane 9: pKK223-3 miniprep from 7/26/11 by Kay Wang Lane 10: ---- Lane 11: pTXB1.His double digest ligation with BSA insert from yesterday Lane 12: pTXB1.His double digest ligation negative control from yesterday Lane 13: pTXB1 double digest ligation with BSA insert from yesterday Lane 14: pTXB1 double digest ligation negative control from yesterday Results