User:Kathryn Muratore/Notebook/AU CHEM-570 lab prep/2011/07/11

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Bench work

  1. Miniprep cultures (done by User:Daniel Catt)
    1. 10 mL LB + 10μL 100 mg/mL Amp + colony from V(His)+I plate from Friday
    2. 10 mL LB + 10μL 100 mg/mL Amp + colony from V(His)+I plate from Friday
    3. 10 mL LB + 10μL 100 mg/mL Amp + colony from V(His)+I plate from Friday
    4. 10 mL LB + 10μL 100 mg/mL Amp + colony from V+I plate from Friday
    5. 10 mL LB + 10μL 100 mg/mL Amp + colony from V+I plate from Friday
    6. 10 mL LB + 10μL 100 mg/mL Amp + colony from V+I plate from Friday
      • 37°C O/N w/ shaking

Results

  • About 10 colonies on V(His)+I plate from Friday
  • About 5 colonies on V+I plate from Friday
    • One colony was separated but the others were clustered near each other