User:Kathryn Muratore/Notebook/AU CHEM-570 lab prep/2011/07/08

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Continue with ligation attempts

Bench work

  1. User:Daniel Catt ran an analytical gel of ligation reactions
  2. Re-plate electroporated DH10B



  • 3 colonies on 400μL plate of NovaBlue transformed with V(His)+I
  • 3 colonies on 100μL plate of electroporated DH10B with V(His)+I
    • 1 colony on negative control (V(His) electroporated into DH10B)


no bands visible for ligation reactions