User:Kathryn Muratore/Notebook/AU CHEM-570 lab prep/2011/07/08

AU CHEM-570 Lab Prep

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Objective Continue with ligation attempts Bench work User:Daniel Catt ran an analytical gel of ligation reactions Re-plate electroporated DH10B User:Daniel Catt plated remainder of all transformations from yesterday Results Transformations 3 colonies on 400μL plate of NovaBlue transformed with V(His)+I 3 colonies on 100μL plate of electroporated DH10B with V(His)+I 1 colony on negative control (V(His) electroporated into DH10B) Gel no bands visible for ligation reactions