User:Karmella Haynes/Notebook/miR Trigger/2011/02/09

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02/09/11

  • ✓ miR sensor transfections: Check for expression; set up selection plates
  • ✓ U2OS: split cells 1:10 and 1:20



miR sensor transfections
> Expression:

  • U2OS plain transfected pretty well. RFP and CFP appear mostly as expected with a quick glance. Set up zeo selection plates (25 ml plates) for all 6 samples
  1. 1/2 total cells + 400 ug/mL zeocin
  2. 1/4 total cells + 400 ug/mL zeocin
  • JDS33-47 mostly dead. Redo transfection with higher plating density next time