User:Karmella Haynes/Notebook/miR Trigger/2011/02/20

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02/20/11

  • ✓ U2OS plain miR sensor lines: pick colonies



U2OS plain miR sensor lines
> Pick RFP positive colonies (4 for each transgene); grow in 24-well plate, 1 mL DMEM/zeo (400 μg/mL) selective medium each
> Colony separation was very good; RFP expression was hard to see, but did see some RFP+ colonies