User:Karmella Haynes/Notebook/Polycomb project/2010/01/12

From OpenWetWare
Jump to: navigation, search
Owwnotebook icon.png Project name Report.pngMain project page
Resultset previous.pngPrevious entry      Next entryResultset next.png

01/12/10

  • ✓ Polycomb-ATF lines: screen for dox-inducible RFP expression
  • ✓ Split U2OS Flp-in T-REx: 1x 1:10 flask



Polycomb-ATF lines
> Check for dox-inducible RFP
--> Each table cell = Dox- / Dox+
--> ✓ = lines kept for further testing

  • - = no RFP
  • + = very few RFP+ cells/ very little expression
  • ++ = about 30 - 60% expression
  • +++ = about 60 - 90% expression
  • ++++ = nearly 100% expression and very bright
  • X = dead cells
Colony: 1 2 3 4 5 6 7 8 9 10 11 12
1. KAH126 +/+++
++/+++ +/+++
-/++
-/++ +/+++ +/++ -/++ +/++ +/+++ +/++ +/+++
2. KAH127 X -/++ +/+++
+/+++
-/++ +/+++ -/++
++/++++ -/++ -/++ +/++ +/+++
3. KAH128 -/++ +/+++
+/++ +/+++ X -/+++
-/+++ -/+++
-/++ +/+++ -/++ ++/++
4. KAH129 -/++ X -/+++
-/+++
-/++ -/++ +/+++ -/+++
-/+++ +/+++ -/+++ +/+++
5. KAH130 -/- -/+
X -/- -/- X -/+
X -/- -/- X X
6. KAH131 -/++
X -/- -/- X -/+
-/- -/+ -/++
X -/- -/-
7. KAH132 -/++ -/+ -/++ -/+++
-/++ -/+++
++/+++ +/+++
-/++ -/++ -/++ -/+
8. KAH133 ++/+++
++/+++
++/++++ ++/++++ +/+++ ++/++++
++/++++ +/+++
bad morph.
++/+++ ++/++++ ++/+++ ++/+++