User:Karmella Haynes/Notebook/Polycomb project/2010/01/12

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01/12/10 ✓ Polycomb-ATF lines: screen for dox-inducible RFP expression ✓ Split U2OS Flp-in T-REx: 1x 1:10 flask Polycomb-ATF lines > Check for dox-inducible RFP --> Each table cell = Dox- / Dox+ --> ✓ = lines kept for further testing - = no RFP + = very few RFP+ cells/ very little expression ++ = about 30 - 60% expression +++ = about 60 - 90% expression ++++ = nearly 100% expression and very bright X = dead cells Colony: 1 2 3 4 5 6 7 8 9 10 11 12 1. KAH126 +/+++ ✓ ++/+++ +/+++ ✓ -/++ ✓ -/++ +/+++ +/++ -/++ +/++ +/+++ +/++ +/+++ 2. KAH127 X -/++ +/+++ ✓ +/+++ ✓ -/++ +/+++ -/++ ✓ ++/++++ -/++ -/++ +/++ +/+++ 3. KAH128 -/++ +/+++ ✓ +/++ +/+++ X -/+++ ✓ -/+++ -/+++ ✓ -/++ +/+++ -/++ ++/++ 4. KAH129 -/++ X -/+++ ✓ -/+++ ✓ -/++ -/++ +/+++ -/+++ ✓ -/+++ +/+++ -/+++ +/+++ 5. KAH130 -/- -/+ ✓ X -/- -/- X -/+ ✓ X -/- -/- X X 6. KAH131 -/++ ✓ X -/- -/- X -/+ ✓ -/- -/+ -/++ ✓ X -/- -/- 7. KAH132 -/++ -/+ -/++ -/+++ ✓ -/++ -/+++ ✓ ++/+++ +/+++ ✓ -/++ -/++ -/++ -/+ 8. KAH133 ++/+++ ✓ ++/+++ ✓ ++/++++ ++/++++ +/+++ ++/++++ ✓ ++/++++ +/+++ bad morph. ++/+++ ++/++++ ++/+++ ++/+++