User:Karmella Haynes/Notebook/Polycomb project/2010/01/11

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01/11/10

  • ✓ Polycomb-ATF lines: split and test w/ doxycycline

Polycomb-ATF lines
> Split each clone into two wells in 12-well dishes; Use zeo/blast/puro maintenance medium (1 mL/well total)
> Use 1μg/mL Dox in second well
> 1 12-well dish per transfection construct KAH126-133 (8 total)
> Check for dox-inducible RFP expression after 24 hours