09/21/12
- ChIP-seq: generate enrichment histogram using DNAStar "Array Star"
Enrichment Histogram
- Start the Array Star software on the Windows platform
- Open project Pc-TF (note: need to write protocol for creating new ChIP-seq project)
- Open the Peak Table
- Select rows to export
- Choose - Data > Export
- Save to: Browse folder (ChIP seq on desktop) and type a file name (PcTF_092112)
- Exported Annotation Fields: click Select, choose all options
- (✓) Export Matching Reads, check box
- Reads Files: Browse Documents > DNASTAR > Genome > Downloads > (appropriate gbk file)
- File format: FASTQ (with quality scores)
- (✓) Launch NGen afterwards, check box
- Error: Find reads mer matches. Finding matches (141 Mbases, 0.00100 k reads processed). Values exceeded storge bit allocation (Close)
- Find new file name.gbk in ChIP seq
- Start the Seq Man NGen software
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