08/25/15
- Cultures - CRISPR library 5 mL cultures
CRISPR library 5 mL cultures
- Re-grow cultures for clones with failed sequencing runs
- From DNASU Order 10294
- Note: DNASU sampling from the 96-well block is top to bottom, then left to right
- A1 = L34-001, A2 = L34-002, A3 = L34-003, etc.
- Low phred scores (<700) generally corresponded with the lowqual reads observed in Benchling
- Scrape bacteria from the original spot agar plates; grow as 5 mL culture for miniprep (overnight)
Clone, well (agar spot position), phred score
- L34-003, C1, 313
- L34-015, G2, 281
- L34-027, C4, 399
- L34-032, H4, 339
- L34-040, H5, 494
- L34-050, B7, 303
- L34-055, G7, 563
- L34-071, G9, 237
- L34-075, C10, 300
- L34-078, F10, 647
- L34-080, G10, 495
- L34-082, B11, 308
- L34-084, D11, 498
- L34-093, E12, 8
- L34-094, F12, 611
- L34-096, H12, 814
RESULTS (08/26/15)
- L34-032
- L34-040
- L34-094
- L34-096
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