User:Karmella Haynes/Notebook/BioBrick cloning/2011/07/08

Project name

07/08/11

✓ Cultures/minipreps: KAH204-209 (2 each)
✓ Site directed mutagenesis: p65 mut DpnI digest & transformation (continued from 7/07/11)

Minipreps
> Check with E/P digests

Reagent	Volume	Expected: 1,2. KAH204 = 2328 3,4. KAH205 = 1251 5,6. KAH206 = 1551 7,8. KAH207 = 1836 9,10. KAH208 = 1884 11,12. KAH209 = 1932	15 μL/lane; 1% agarose
DNA(plasmid)	2.0 μL
10X buffer	1.5
EcoRI	1.0
PstI	1.0
dH₂O	9.5
	15 μL --> 37°C/ ~15 min.