User:Karmella Haynes/Notebook/BioBrick cloning/2011/06/04

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06/04/11

  • ✓ Cultures/minipreps: Single miR sensor genes in MV8 (2 each); do digests tomorrow
  • ✓ 6/05/11 Digests



Minipreps
> Check with E/ApaLI
> mCherry has ApaLI site* (will cut within RFP-Repressor genes, see 1/20/11)

Reagent Volume Expected:
MV8 bands = 1243, 1135, 288, 234

CFP inserts + 1655
1,2. KAH186/MV8 = 3680
3,4. KAH184/MV8 = 3846
5,6. KAH187/MV8 = 3924
7,8. KAH188 = 3779
9,10. KAH189 = 3951
11,12. KAH190 = 4023
13,14. KAH191 = 3878
15,16. KAH183 = 4038
17,18. KAH192 = 4116

RFP inserts, last frag. + 1655**
19,20. KAH196 = 1437*, 711*, 2772**
21,22. KAH198 = 1681*, 711*, 2772**
File:KAH 060511 gel1.tif
15 μL/lane; 1% agarose
DNA(plasmid) 2.0 μL
10X buffer 1.5
EcoRI 1.0
ApaLI 1.0
dH2O 9.5
  15 μL --> 37°C/ ~15 min.