06/04/11
- ✓ Cultures/minipreps: Single miR sensor genes in MV8 (2 each); do digests tomorrow
- ✓ 6/05/11 Digests
Minipreps
> Check with E/ApaLI
> mCherry has ApaLI site* (will cut within RFP-Repressor genes, see 1/20/11)
Reagent |
Volume
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Expected: MV8 bands = 1243, 1135, 288, 234
CFP inserts + 1655 1,2. KAH186/MV8 = 3680 3,4. KAH184/MV8 = 3846 5,6. KAH187/MV8 = 3924 7,8. KAH188 = 3779 9,10. KAH189 = 3951 11,12. KAH190 = 4023 13,14. KAH191 = 3878 15,16. KAH183 = 4038 17,18. KAH192 = 4116
RFP inserts, last frag. + 1655** 19,20. KAH196 = 1437*, 711*, 2772** 21,22. KAH198 = 1681*, 711*, 2772**
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15 μL/lane; 1% agarose
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DNA(plasmid) |
2.0 μL
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10X buffer |
1.5
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EcoRI |
1.0
|
ApaLI |
1.0
|
dH2O |
9.5
|
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15 μL --> 37°C/ ~15 min.
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