User:Karmella Haynes/Notebook/BioBrick cloning/2010/08/24

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08/24/10

  • ✓ Cultures: pick 6 colonies from phGluc streak plate; grow in 50 μg/mL Zeocin low salt LB (5 mL)



Cultures
> Andrew's Zeo expression test showed that low salt LB is required for zeocin to be effective
> Low salt LB:

  • 2.5 g NaCl
  • 2.5 g Yeast extract
  • 5.0 g Tryptone
  • pH 7.5

> Andrew also showed that 50 μg/mL Zeocin is sufficient for killing non-resistant bacteria