User:Karmella Haynes/Notebook/BioBrick cloning/2010/08/24

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08/24/10 ✓ Cultures: pick 6 colonies from phGluc streak plate; grow in 50 μg/mL Zeocin low salt LB (5 mL) Cultures > Andrew's Zeo expression test showed that low salt LB is required for zeocin to be effective > Low salt LB: 2.5 g NaCl 2.5 g Yeast extract 5.0 g Tryptone pH 7.5 > Andrew also showed that 50 μg/mL Zeocin is sufficient for killing non-resistant bacteria