User:Karmella Haynes/Notebook/BioBrick cloning/2010/08/09

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08/09/10 ✓ Minipreps: KAH160-170/KAH015 (1 each) ✓ Cultures: KAH160-170/KAH015 25 mL cultures for midipreps Minipreps > Check with KpnI digests Reagent Volume Expected: Backbone = 7489 Empty vector frag. = 913 1. KAH160/KAH015 = 2060 2. KAH161/KAH015 = 2060 3. KAH162/KAH015 = 2060 4. KAH163*/KAH015 = 710, 2319 *Has a KpnI site 5. KAH164/KAH015 = 3047 6. KAH165/KAH015 = 1904 7. KAH166/KAH015 = 1904 8. KAH167/KAH015 = 1904 9. KAH168*/KAH015 = 710, 2163 *Has a KpnI site 10. KAH169/KAH015 = 2871 11. KAH170/KAH015 = 1868 15 μL/lane; 1% agarose DNA(plasmid) 2.0 μL 10X buffer 1.5 KpnI 1.0 dH2O 10.5   15 μL --> 37°C/ ~15 min.