User:Karmella Haynes/Notebook/BioBrick cloning/2010/01/17

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01/17/10

  • ✓ Cultures: KAH118/MV2 (from re-transformation plate, 4)
  • ✓ Minipreps: KAH137(3), 138(3)

Minipreps
> Check with E/P digests

Reagent Volume Expected:
v0120 = 3200
1-3. KAH137 = 2393
3,4. KAH138 = 1981
 E/P digest 1/17/10
15 μL/lane; 1% agarose
DNA(plasmid) 2.0 μL
10X buffer 1.5
EcoRI 1.0
PstI 1.0
dH2O 9.5
  15 μL --> 37°C/ ~15 min.



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